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Title   위선암세포에서 세포내 Cyclic AMP 의 증가가 Epidermal Growth Factor 수용체결합에 미치는 영향 ( Effect of Interacellular Cyclic AMP on EGF Receptor Binding in Human Gastric Adenocarcinoma Cells )
Publicationinfo   1993 Jan; 025(02): 166-170.
Key_word   Cyclic AMP, Epidermal growth factor receptor, Gastric cancer cell line
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Abstract   Different types of cyclic AMP(cAMP) analogues and the agents increasing intracellular cAMP have growth inhibitory effect on epithelial origin cancer cell lines including gastric can- cer. Transforming growth factor a(TGFa) is a passible autocrine growth factor of normal and neoplastic epithelial cells, since it has been shown that TGFa and its receptor(EGF receptor: EGFr) are expressed in those cells, growth of which are stimulated by TGFa. The purpose of our study is to find whether increasing intracellular cAMP has any effect on EGFr binding. AGS human gastric cancer cell line was used because AGS cells express TGFa and its receptor and TGFa/EGF stimulates and cAMP inhibits the growth of AGS. Vasoactive intestinal polypeptide(VIP)(10 M) plus isobutylmethylxanthine(IBMX; 10(-3)M) stimulated cAMP production in AGS cells. AGS cells has a high-affinity single type EGFr and 24 hour treatment of these a- gents decreased the binding capacity(Bmax: control vs treated; 7.27 fmole/10(5) cells vs 5.41 fmole/10(5) cells) and increased affinityKd: control vs treated: 80.8 fM vs 39.6 fM), which is representative data from triple experiments. In conclusion, increased intracellular cAMP affects EGFr binding on the AGS cell membrane and we need to investigate further whether this phe- nomenon is related to the effect an cell growth.
저 자   김선회(Sun Whe Kim),김진복(Jin Pok Kim)